Bertolini is an Italian surname. Notable people with the surname include:

• Adrián Bertolini (born 1978), Uruguayan basketball player
• Alessandro Bertolini (born 1971), cyclist
• Andrea Bertolini (born 1973), race car driver
• Christopher Bertolini, American film writer and producer
• Gioele Bertolini (born 1995), Italian cyclist
• Jack Bertolini (1934–2021), Scottish footballer
• José Elguero Bertolini (born 1934), Spanish chemist
• Luigi Bertolini (1904–1977), football player
• Mark Bertolini (born 1956), American business executive
• Massimo Bertolini (born 1974), Italian tennis player
• Michelle Bertolini (born 1994), Venezuelan tennis player
• Milena Bertolini (born 1966), Italian football player and manager
• Pietro Bertolini (1859–1920), Italian statesman
• Roberto Bertolini (born 1985), Italian javelin thrower
• Veronica Bertolini (born 1995), Italian gymnast