Jules Buysse (Wontergem, 13 August 1901 – 31 December 1950) was a Belgian racing cyclist.
Buysse was the youngest of three brothers who competed in the Tour de France. He won the first stage of 1926 Tour de France by 13 minutes and 6 seconds, allowing him to wear the yellow jersey for two days until losing it to fellow Belgian Gustave Van Slembrouck. He finished the tour in 9th place.
His brothers were Lucien Buysse, who won the 1926 Tour de France, and Marcel Buysse who won six stages on the 1913 Tour.