Guido De Rosso (born 28 September 1940) is a retired Italian professional racing cyclist. He won several races in the 1960s and finished seventh at the 1965 Tour de France. He rode the Giro d'Italia in 1962–1965 and finished fourth in 1963 and third in 1964.

He was the first rider to get a professional victory while riding a Pinarello bicycle. It was two stages of the Tour de l'Avenir as well as winning overall.

To celebrate turning 80 Mattia Perencin, the mayor of Farra di Soligo, presented De Rosso with a plaque of his achievements.